Because Doppler signal is vulnerable to impact of the surrounding environment,its frequency is difficult to analyze with general digital signal processing methods.The mixed programming of Burg spectral estimation algorithm was carried out with LabVIEW and MATLAB,and a simple speed testing system which consists of Doppler speed sensor,USB-6215 DAQ(Data-Acquisition) made by National Instruments and PC was experimented.The experimental results showed that the system is feasibility and accuracy.