Bacteria column leaching and acid column leaching experiments of a hard uranium ore were carried out.The results of the experiments were compared and studied.And it is showed that,compared with acid column leaching,the acid consumption of bacteria column leaching was reduced by 0.5%,meanwhile,the leaching rate of uranium was increased by 8%,the leaching period was shorten by 10 days at least.The experiments showed that the bacteria leaching of this uranium ore is feasibility.