Bacteria column leaching and acid column leaching of a uranium ore from a mine were tested and compared.The results showed that compared with acid column leaching,the acid consumption of bacteria column leaching was reduced by 0.9%,the leaching rate of uranium was increased by 9%,the leaching period was shorten by19 days.It is showed that bacteria leaching can leach the uranium which can’t been leached by conventional acid leaching.