The separation experiment of a phosphorus ore had be carried out with the purpose of removing rocky minerals. This phosphorus ore mostly composes of carbonate minerals, and the main rocky minerals are dolomite, calcite, quartz, etc. Through the kinetic analysis of the grinding process, the grind fineness had be determined; the reverse flotation experiment of the ore had be carried out with small-size flotation cell, and the optimal reagent system had be determined. The experimented results showed that the qualities of the prepared phosphorus ore could meet with the quality requirements of market on phosphorus ore at present.